The AmOnco88™ Comprehensive Cancer Panel targets specific cancer genes to identify tumor-specific sequence mutations, micro-satellite instability (MSI), amplifications and genomic rearrangements using proprietary bioinformatics software.

As part of the PROGENEUS™ platform, AmOnco88™ analyzes 88 well-characterized cancer genes from tumors (frozen or FFPE). AML’s proprietary methods can accommodate low tumor purity samples.

The analysis includes:

  • Complete coding region analysis of 76 genes for single nucleotide variants, insertions, deletions
  • Copy number analysis of 13 well-characterized cancer genes
  • Rearrangement analysis of 14 well-characterized cancer genes
  • Microsatellite instability analysis of 5 markers

List of genes

Sequence analyses for 76 well-characterized cancer genes (SNVs, Insertions, Deletions)

ABL1 ERBB4 GNAQ MTOR RET
AKT1 EZH2 GNAS NF1 ROS1
ALK FANCA HNF1A NF2 SMAD4
APC FANCC HRAS NOTCH1 SMARCB1
ATM FANCD2 IDH1 NPM1 SMO
BRAF FANCE IDH2 NRAS SRC
BRCA1 FANCF JAK2 NTRK1 STK11
BRCA2 FANCG JAK3 PALB2 TERT
BRIP1 FANCL KDR PDGFRA TP53
CDH1 FBXW7 KIT PDGFRB TSC1
CDKN2A FGFR1 KRAS PIK3CA TSC2
CSF1R FGFR2 MET PMS2 VHL
CTNNB1 FGFR3 MLH1 PTCH1
DDR2 FLT3 MPL PTEN
EGFR FOXL2 MSH2 PTPN11
ERBB2 GNA11 MSH6 RB1

Copy number analyses

ALK ERBB3 FGFR3 MYC RET
EGFR FGFR1 KIT MYCN
ERBB2 FGFR2 MET PDGFRA

Rearrangement analyses

ALK EGFR ETV6 PDGFRA ROS1
BCL2 ETV1 EWSR1 PDGFRB TMPRSS2
BCR ETV4 MLL RARA

Microsatellite analyses

BAT-25 BAT-26 MONO-27 NR-21 NR-24

Test Specifications

Regions Analyzed Coding regions of 76 genes and selected regions of 14 genes (Amplifications/Rearrangements)
Sequencing method Hybridization-capture followed by Illumina Next Generation Sequencing
Sequencing depth >500X
Assay Sensitivity and Specificity >99%
Sample requirement Tumor with matched normal; Tumor only
Sample type FFPE tumor tissues; blood or saliva for matched normal
Turn-around time 2 weeks
Variant frequency >2%